Fluorescence Detector

The Ultrafluor Fluorescence Detector is a highly sensitive, scanning fluorescence detector for liquid chromatography. Its dual monochromator design provides exceptional optical performance and operational flexibility for routine and trace analyses.

High performance

An improved optical design provides exceptional performance. High-efficiency holographic diffraction gratings are optimized for high sensitivity at both lower and higher excitation wavelengths.

Customize your selectivity by choosing either of three spectral bandwidths. The pulsed xenon lamp provides a high energy level across the entire UV/Vis spectrum and produces no harmful ozone. Fluctuations in lamp intensity are automatically maintained and corrected to reduce noise and drift.

Quantitative accuracy and precision are greatly enhanced by the use of 20-bit digital electronics, producing a wider dynamic range. The electronics design allows emission monitoring of fluorescence, phosphorescence, and chemiluminescence.

Improved laboratory productivity

The Ultrafluor makes your job easier. Time programming of the Ex l and Em l lets you optimize sensitivity and selectivity. Automatic, real-time scanning of eluting peaks helps you identify optimum wavelengths. No more time-consuming manual stop-flow techniques.

Saves setup time

Create up to four user files with ten timelines each for fast, easy wavelength changes. All files can be linked with the built-in queue.

A unique feature allows the emission data of any one of up to sixty stored spectra to be displayed on the LCD or played back to an external data collection device.

The signal response can be normalized to any compound of interest, or a preset factory response factor can be used. This provides consistent unit-to-unit response.

Easy access and maintenance

The lamp and flow cell are prealigned and easily accessible from the front of the instrument. Exchange or service is quick and easy. A separate lamp and flow cell housing assure quick maintenance.

Flow cell

The Ultrafluor includes a biocompatible flow cell with 8 µL illuminated volume.

Optical design	Dual monochromators using concave holographic diffraction gratings; stepper-motor driven
Sensitivity	S/N >1500 for 2 µg/L anthracene in MeOH; 248 nm Ex/398 nm Em
Wavelength range	200 to 650 nm excitation and emission; 200 to 800 nm emission with optional extended-range (red-sensitive) PMT
Wavelength accuracy	±2 nm @248 nm Ex/398 nm Em
Wavelength precision	<0.5 nm
Spectral bandwidth	8, 20, or 30 nm; selectable
Lamp	Pulsed xenon; selectable 20 or 100 Hz
Flow cell	High-purity quartz, Teflon®, Kalrez®, and PEEK; 8 µL illuminated volume; maximum pressure: 200 psi (14 bar)
Spectral scanning	Automatic or manual; 100 steps per second; step size selectable; 2, 4, 8, 16, or 32 nm; up to 60 spectra stored in memory*
Range Selections	500, 200, 100, 50, 20, 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05, 0.02, 0.01 FUFS
Operating modes	Fluorescence; phosphorescence; chemiluminescence
Analog outputs	2, Range-selectable over entire fluorescence range using 20-bit D/A
Fluorescence range	0.01-500 fluorescence units full scale
Method files	4 user files protected in nonvolatile memory; files can be linked via queue; 10 time lines per file for wavelength changes; automatic zero
Ambient environment	10-40° C, 5-95% relative humidity, noncondensing
Communication	Remote inputs: Run, Stop, Zero Output: Ready
Display	2 lines x 24 characters, high-contrast LCD
Dimensions	17 cm x 30 cm x 40 cm (H x W x D) 7" x 12" x 16"
Weight	11 kg (24 lbs.)
Power	100/120, 220/240 Vac nominal; 50/60 Hz; 2 Amps max

* For scans of 100 nm with a 2-nm interval (50 discrete data points)

Teflon® is a registered trademarks of E. I. duPont de Nemours, Inc.
Kalrez® is a registered trademark of DuPont Dow Elastomers.