The Series 2500 Protein LC System, built around the Series 2500 integrated quaternary low-pressure mixing pump, is a compact HPLC package that gives you the capability to perform protein separations and routine analytical chromatography applications for a fraction of the cost of other systems.

The Model V520 UV/VIS detector gives you the sensitivity and flexibility today’s separations require. The fixed wavelength 254/280nm detector is an economical option. The system is available with Stainless steel or PEEK fluid paths. The Series 2500 Gradient System can be operated in stand-alone mode or through PC software. The Series 2500 pump stores up to 10 methods and allows interfacing with autosamplers and data systems for system automation.

Data collection can be achieved by adding a chart recorder or PeakSimple data acquisition software.

The Series 2500 pump can store up to ten gradient methods. Each method may be up to 50 steps long with a total run time of 999.99 minutes. Each step can be programmed with the solvent composition, flow rate, and external events to provide extended HPLC system control and automation capability. Methods may be automatically repeated up to 99 times, and linked to occur in a user-programmed sequence. The dual piston 12mL pump in the Model 2500 provides compact and fully integrated modern stepper motor technology combined with years of industry experience to create a high performance system at an exceptional value.

Pump performance is optimized with a vacuum degasser. For preparative applications where high flow rates are required, order the 100 mL version of the Model 2500 pump.

PeakSimple Data Acquisition

• Collect data using single-channel or 4-channel PeakSimple Data package.

• Easy to learn, easy to use software-produce results almost immediately.

• Simple user interface features for manual integration, baseline subtraction and data smoothing, multi-level calibration, overlaying chromatograms, data logger mode.

• Easy to connect interface box hooks up to most standard Pentium PCs running Windows.

• Built-in timing mechanism to work with Windows applications (Excel, Word).

UV-Vis Detector

The Model V520 is one of the most versatile, reliable, and sensitive UV-Vis absorbance monitors available. The V520 uses state-of-the-art photodiode design with complementary high-photon throughput optics. With a variety of sample cell designs, this detector is capable of meeting virtually every application requirement in the laboratory.

• The V520 Variable UV-Vis detector is capable of working from 190 to 400 nm with optical range extension into the NIR (940 nm) with broad bandpass, using only the D2 lamp.

• The V520 offers one of the lowest noise and drift specifications in the industry.

• The V520 can be modified in the field without special alignment tools to accommodate many different sample cells. Available sample cells include analytical, microbore and preparative. For preparative protein separations, use either the Prep variable path length (0-4 mm) flow cell or the fixed pathlength (1 mm) cell.

• An economical detector option is the fixed wavelength 254/280nm Model F110 detector. The same flow cell options are available with this version.

Add an autosampler and fraction collector for automation

Variable-volume Autosampler

The variable-volume autosampler provides outstanding performance and maximum flexibility in a variable-volume, LC sample processing system. The standard unit provides 120-vial capacity, multiple injection modes, random vial access, reuse of calibration standards, and nonvolatile storage of 4 complete methods.

Inert Autosampler

The inert autosampler provides all the features of the variable-volume autosampler, but in a metal-free, inert version. This autosampler is ideally suited for biological applications requiring metalfree operation or applications that use corrosive solvents or buffers.

FC Jr. Fraction Collector

FC Jr. holds microcentrifuge tubes to 250 mL Erlenmeyers, and easily stretches to hold tubes up to 150 mm tall. FC Jr. gives you great capacity in minimal space. FC racks are available for almost any tube or vial, and even 96-well microplates. When you need increased amounts of purified product, FC Jr. lets you collect unlimited volumes in off-deck containers with a funnel rack.

Standard Features of FC Jr.

• Collect peaks of interest using any combination of slope, level, and time windows.

• Optional interface cable for connection to detector – used for slope/level sensing, event marking, start and advance inputs.

• Collect uniform fractions by time, drop count, or pumped volume.

act system re